1SHY
The Crystal Structure of HGF beta-chain in Complex with the Sema Domain of the Met Receptor.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.928 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 137.052, 186.352, 66.659 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.220 |
R-factor | 0.21206 |
Rwork | 0.209 |
R-free | 0.26978 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HGF beta chain |
RMSD bond length | 0.012 |
RMSD bond angle | 1.537 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 28312 | |
Completeness [%] | 99.4 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | PEG, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |