1SHW
EphB2 / EphrinA5 Complex Structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.890, 88.970, 122.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
Rwork | 0.258 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EphB2/ EphrinB2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | |
Number of reflections | 21147 | |
<I/σ(I)> | 15.8 | |
Completeness [%] | 98.6 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 278 | PEG 3350, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |