1SGU

Comparing the Accumulation of Active Site and Non-active Site Mutations in the HIV-1 Protease

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 61
格子定数 [Å]	61.573, 61.574, 84.109
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	19.600 - 1.900
R因子	0.22
Rwork	0.220
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	1.261
結合角の平均二乗偏差(RMSD) [度]	3.300
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	19.600	1.970
分解能 [Å] (高)	1.900	1.900
独立反射数	14298
<I/σ(I)>	9.7
完全性 [%]	96.6	80.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		4.5		20 mM Sodium acetate, 1.5 M ammonium Sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K, pH 4.50