1SEU
Human DNA Topoisomerase I (70 Kda) In Complex With The Indolocarbazole SA315F and Covalent Complex With A 22 Base Pair DNA Duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-01 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.382, 115.965, 73.547 |
Unit cell angles | 90.00, 93.71, 90.00 |
Refinement procedure
Resolution | 46.630 - 3.000 |
Rwork | 0.237 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4t |
RMSD bond length | 0.012 |
RMSD bond angle | 1.500 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNX (2002) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 15533 | |
<I/σ(I)> | 15.4 | 2.5 |
Completeness [%] | 80.5 | 38.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 289 | PEG 8000, AMMONIUM SULFATE, MES, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | AMMONIUM SULFATE | ||
3 | 1 | 1 | MES | ||
4 | 1 | 2 | AMMONIUM SULFATE | ||
5 | 1 | 2 | MES |