1SEH
Crystal structure of E. coli dUTPase complexed with the product dUMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8040 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 75.257, 75.257, 100.532 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.470 |
R-factor | 0.15895 |
Rwork | 0.158 |
R-free | 0.18384 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1euw |
RMSD bond length | 0.021 |
RMSD bond angle | 1.379 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.600 |
High resolution limit [Å] | 1.470 | 1.470 |
Number of reflections | 29251 | |
<I/σ(I)> | 27.6 | 4.86 |
Completeness [%] | 99.9 | 100 |
Redundancy | 31.8 | 20.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 3350, SODIUM ACETATE, TRIS, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |