1SD8
ARSENATE REDUCTASE R60K MUTANT FROM E. COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 86.600, 86.600, 116.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.590 |
Rwork | 0.140 |
R-free | 0.18120 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | NATIVE STRUCTURE |
RMSD bond length | 0.011 |
RMSD bond angle | 0.030 |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.104 | 0.234 |
Number of reflections | 32216 | |
<I/σ(I)> | 25.9 | 3.88 |
Completeness [%] | 95.0 | 69.2 |
Redundancy | 16.9 | 3.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 278 | 50% saturated Cesium Sulfate, 100mM sodium acetate, 5mM DTT, pH 4.80, VAPOR DIFFUSION, HANGING DROP, temperature 278K |