1SC6
Crystal Structure of W139G D-3-Phosphoglycerate dehydrogenase complexed with NAD+
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-23 |
| Detector | ANL-ECT |
| Wavelength(s) | 0.97934, 0.97951, 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.487, 70.842, 149.468 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.090 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.070 |
| Rmerge | 0.078 | 0.407 |
| Number of reflections | 104640 | |
| <I/σ(I)> | 20.6 | 2 |
| Completeness [%] | 95.9 | 95.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | Citrate, PEG 4K, NAD, alpha-ketoglutarate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
