1SAZ
Membership in the ASKHA Superfamily: Enzymological Properties and Crystal Structure of Butyrate Kinase 2 from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9799, 0.9796, 0.9574 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 197.685, 197.685, 58.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.640 - 2.500 |
| Rwork | 0.217 |
| R-free | 0.26700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 17673 | |
| <I/σ(I)> | 15.9 | 2.67 |
| Completeness [%] | 87.7 | 65.6 |
| Redundancy | 11.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | n-octyl-beta-D-glucoside, Tris-HCl pH 8.5, NaCl, DTT, glycerol, ADP, MgCl2, isobutyrate, sodium formate, acetate. AMPPCP, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
