1SA1
Tubulin-podophyllotoxin: stathmin-like domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-10 |
Detector | ADSC |
Wavelength(s) | 0.933 |
Spacegroup name | P 65 |
Unit cell lengths | 328.061, 328.061, 54.301 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 4.200 |
R-factor | 0.20643 |
Rwork | 0.204 |
R-free | 0.25935 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sa0 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.091 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.300 |
High resolution limit [Å] | 4.200 | 4.200 |
Rmerge | 0.060 | 0.482 |
Number of reflections | 24624 | |
<I/σ(I)> | 18 | 2 |
Completeness [%] | 98.3 | 90.2 |
Redundancy | 3.26 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | Gigant, B., (2000) Cell, 102, 809. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG |