1S9K

Crystal Structure of Human NFAT1 and Fos-Jun on the IL-2 ARRE1 Site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1996-09-16
Detector	BRANDEIS - B4
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	64.769, 86.260, 84.044
Unit cell angles	90.00, 111.24, 90.00

Refinement procedure

Resolution	30.000 - 3.100
R-factor	0.2423
Rwork	0.242
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.200
High resolution limit [Å]	3.100	3.100
Number of reflections	16172
Completeness [%]	96.9	71.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	300-400 mM Ammonium Acetate Salt, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Ammonium Acetate Salt
2	1	1	H2O
3	1	2	Ammonium Acetate Salt
4	1	2	H2O