1S9C
Crystal structure analysis of the 2-enoyl-CoA hydratase 2 domain of human peroxisomal multifunctional enzyme type 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-06-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9090 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.295, 105.431, 206.944 |
Unit cell angles | 90.00, 103.37, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
Rwork | 0.229 |
R-free | 0.26460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pn2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.409 |
Data reduction software | DENZO |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.200 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.096 | 0.230 |
Number of reflections | 77386 | |
<I/σ(I)> | 14.2 | 4.6 |
Completeness [%] | 93.7 | 70.2 |
Redundancy | 3.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | PEG 10000, HEPES, MnCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | PEG 10000, HEPES, MnCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |