1S8C
Crystal structure of human heme oxygenase in a complex with biliverdine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 119 |
| Detector technology | CCD |
| Collection date | 2002-10-12 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.413, 56.461, 109.751 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.190 |
| Rwork | 0.240 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qq8 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.062 | 0.044 |
| Number of reflections | 55646 | |
| <I/σ(I)> | 17.3 | 2.1 |
| Completeness [%] | 86.5 | 90.2 |
| Redundancy | 8.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 301 | ammonium sulfate, hepes, 1,6 hexanediol, water, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 301K |
