1S8A

H98Q Mutant of Methylglyoxal Synthase from E. coli complexed with Phosphoglycolic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2002-04-11
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	53.076, 129.960, 178.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.650 - 2.200
Rwork	0.192
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.650	2.240
High resolution limit [Å]	2.200	2.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	PEG 1500, Sodium Cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K