1S72
REFINED CRYSTAL STRUCTURE OF THE HALOARCULA MARISMORTUI LARGE RIBOSOMAL SUBUNIT AT 2.4 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 211.660, 299.670, 573.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22200 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.691 |
| Number of reflections | 666819 | |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 95.6 | 73.5 |
| Redundancy | 9.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 292 | PEG 6000, KCL, NH4Cl, MGCL2, pH 5.80, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 292 | PEG 6000, KCL, NH4Cl, MGCL2, pH 5.80, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | NH4Cl | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 6000 | ||
| 7 | 1 | 2 | KCL | ||
| 8 | 1 | 2 | NH4Cl | ||
| 9 | 1 | 2 | MGCL2 |
