1S68
Structure and Mechanism of RNA Ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.717, 89.892, 47.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.000 * - 1.900 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22700 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RESOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.322 |
Total number of observations | 79243 * | |
Number of reflections | 19766 | |
<I/σ(I)> | 18.2 | 2.6 |
Completeness [%] | 97.1 | 83.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 23 * | 100mM Tris, 8.5, 0.2M sodium acetate, 5mM DTT, 28% PEG-3350, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 23 * | 100mM Tris, 8.5, 0.2M sodium acetate, 5mM DTT, 28% PEG-3350, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 100 (mM) | pH8.5 |
2 | 1 | drop | sodium acetate | 0.2 (M) | |
3 | 1 | drop | dithiothreitol | 5 (mM) | |
4 | 1 | drop | PEG3350 | 28 (%) | |
5 | 1 | reservoir | glycerol | 20 (%) |