1S68
Structure and Mechanism of RNA Ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.717, 89.892, 47.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 * - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22700 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.322 |
| Total number of observations | 79243 * | |
| Number of reflections | 19766 | |
| <I/σ(I)> | 18.2 | 2.6 |
| Completeness [%] | 97.1 | 83.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 23 * | 100mM Tris, 8.5, 0.2M sodium acetate, 5mM DTT, 28% PEG-3350, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 23 * | 100mM Tris, 8.5, 0.2M sodium acetate, 5mM DTT, 28% PEG-3350, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 100 (mM) | pH8.5 |
| 2 | 1 | drop | sodium acetate | 0.2 (M) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | PEG3350 | 28 (%) | |
| 5 | 1 | reservoir | glycerol | 20 (%) |
