1S5L
Architecture of the photosynthetic oxygen evolving center
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 134.992, 228.849, 309.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.500 |
R-factor | 0.296 |
Rwork | 0.296 |
R-free | 0.34600 * |
Structure solution method | MIR |
RMSD bond length | 0.014 |
RMSD bond angle | 2.110 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 3.520 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.080 * | 0.430 * |
Total number of observations | 298731 * | |
Number of reflections | 103604 | |
Completeness [%] | 87.3 * | 80.9 |
Redundancy | 2.88 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * | 18 * | 100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | chlorophyll | 1.75 (mg/ml) | |
2 | 1 | drop | MES | 20 (mM) | pH6.5 |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | 10 (mM) | ||
5 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
6 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
7 | 1 | reservoir | glycerol | 20 (%) | |
8 | 1 | reservoir | PEG4000 | 8.0-14.0 (%) | |
9 | 1 | reservoir | 0.11 (mM) |