1S50
X-ray structure of the GluR6 ligand binding core (S1S2A) in complex with glutamate at 1.65 A resolution
Experimental procedure
| Experimental method | Single Wavelength 0.97946 |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-27 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 61 |
| Unit cell lengths | 52.272, 52.272, 169.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.130 - 1.650 |
| R-factor | 0.2013 |
| Rwork | 0.200 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1S7Y (rcsb021380) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.355 |
| Number of reflections | 31253 | |
| <I/σ(I)> | 13.1 | 3.82 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 24% PEG 4000 2 TRIS 20 NaCl 1 EDTA 10 NaGlu , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
