1S4Y
Crystal structure of the activin/actrIIb extracellular domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.290, 120.627, 43.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.710 - 2.300 |
R-factor | 0.21021 |
Rwork | 0.206 |
R-free | 0.28615 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.031 |
RMSD bond angle | 2.385 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 19028 | |
Completeness [%] | 94.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | PEG 3350, magnesium chloride, bis tris, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |