1S4O
Crystal structure of yeast alpha1,2-mannosyltransferase Kre2p/Mnt1p: binary complex with GDP/Mn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.694, 101.068, 62.036 |
Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
Resolution | 52.420 - 2.010 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s4n |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.056 | 0.251 |
Number of reflections | 49240 | |
<I/σ(I)> | 15 | 3.7 |
Completeness [%] | 96.7 | 98.8 |
Redundancy | 2.11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | GDP, manganese chloride, PEG 2000 MME, HEPES, SODIUM CHLORIDE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |