1S4E
Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97950, 0.97973, 0.93928 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 211.974, 355.672, 165.613 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.23215 |
| Rwork | 0.230 |
| R-free | 0.26972 |
| Structure solution method | MAD |
| Starting model (for MR) | MAD phased model |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.659 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 137024 | |
| <I/σ(I)> | 17.81 | 3.4 |
| Completeness [%] | 99.5 | |
| Redundancy | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | Ammonium sulphate, Tris-HCl, galactose, ADP, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
