1S3Z
Aminoglycoside N-Acetyltransferase AAC(6')-Iy in Complex with CoA and Ribostamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.989, 44.688, 87.974 |
| Unit cell angles | 90.00, 93.86, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.980 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 20976 | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 93.0 | 67.6 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | Peg 3350, ammonium sulfate, triethanolamine, coenzyme A, ribostamycin, pH 8.0, vapor diffusion under oil, temperature 293K, pH 8.00 |
