1S3X
The crystal structure of the human Hsp70 ATPase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 120 |
| Detector technology | AREA DETECTOR |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 145.470, 63.340, 45.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.840 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1atr |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 1.890 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.049 | 0.020 |
| Number of reflections | 35081 | 3392 * |
| <I/σ(I)> | 28.3 | |
| Completeness [%] | 95.5 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 10 * | imidazole buffer, PEG 8000, CaCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | reservoir | imidazole | 25 (mM) | pH7.0 |
| 3 | 1 | reservoir | PEG8000 | 8 (%) | |
| 4 | 1 | reservoir | 20 (mM) | ||
| 5 | 1 | reservoir | gamma-S-ATP | 1 (mM) |
