1S3D
ARSENATE REDUCTASE R60A MUTANT FROM E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-24 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.600, 86.600, 115.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.540 |
| R-factor | 0.1466 |
| R-free | 0.19850 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | NATIVE STRUCTURE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.030 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.588 |
| High resolution limit [Å] | 1.529 | 1.529 |
| Rmerge | 0.093 | 0.297 |
| Number of reflections | 34567 | |
| <I/σ(I)> | 25.1 | 3.13 |
| Completeness [%] | 92.6 | 56.6 |
| Redundancy | 16.38 | 3.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 50% Saturated Cesium sulfate, 100 mM sodium acetate, 5 mM DTT, pH 4.80, VAPOR DIFFUSION, HANGING DROP |
