1S36
Crystal structure of a Ca2+-discharged photoprotein: Implications for the mechanisms of the calcium trigger and the bioluminescence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-20 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.445, 53.445, 144.032 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.960 |
| R-factor | 0.22459 |
| Rwork | 0.223 |
| R-free | 0.25922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jf2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.207 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | LSCALE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.460 | 2.050 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 15775 | |
| <I/σ(I)> | 9.37 | 2.11 |
| Completeness [%] | 1.0 | 0.97 |
| Redundancy | 14.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 7.5 | 277 | 1.5 M tri-sodium citrate, 0.1 M Na-HEPES, pH 7.5, modified microbatch, temperature 277K |
