1S1L
Influence of Groove Interactions on the Formation of DNA Holliday Junctions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.542 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.923, 24.869, 37.095 |
Unit cell angles | 90.00, 109.22, 90.00 |
Refinement procedure
Resolution | 18.200 - 2.200 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ndb id UD0019 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.800 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.200 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 | 0.291 |
Number of reflections | 3492 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 93.4 | 61.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 25mM Na Cacodylate, .5mM DNA, 180mM CaCl2, 2.4mM spermine against 20% resevoir MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Na Cacodylate | ||
2 | 1 | 1 | .5mM DNA | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 1 | spermine | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | Na Cacodylate |