1S1E

Crystal Structure of Kv Channel-interacting protein 1 (KChIP-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	143
Detector technology	CCD
Collection date	2000-08-26
Detector	ADSC QUANTUM 4
Wavelength(s)	1.45
Spacegroup name	P 41 21 2
Unit cell lengths	50.133, 50.133, 177.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.25
Rwork	0.224
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bjf
RMSD bond length	0.006
RMSD bond angle	1.117
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	10497
<I/σ(I)>	25.6
Completeness [%]	99.9	95
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	291	PEG3350,Calcium chloride, Ammonium Chloride, DTT, MES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K