1RXU
E. coli uridine phosphorylase: thymidine phosphate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 156.244, 97.655, 161.453 |
| Unit cell angles | 90.00, 118.23, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.100 |
| R-factor | 0.21256 |
| Rwork | 0.212 |
| R-free | 0.22945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A SINGLE HEXAMER (CHAINS ABCDEF) OF THE 5-FLUOROURACIL PHOSPHATE UP COMPLEX MODEL. |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.162 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.890 | 3.260 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.158 | 0.529 |
| Number of reflections | 76410 | |
| <I/σ(I)> | 4.7 | 1.4 |
| Completeness [%] | 98.2 | 97 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH METHOD UNDER OIL | 7.5 | 289 | TRIS HCL, PEG4000, POTASSIUM ACETATE, THYMIDINE, POTASSIUM PHOSPHATE, pH 7.50, BATCH METHOD UNDER OIL, temperature 289K |
