1RXD
Crystal structure of human protein tyrosine phosphatase 4A1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-06-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97911, 0.97934, 0.97166 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.291, 105.568, 179.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.590 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.132 | 0.575 |
| Number of reflections | 48954 | |
| <I/σ(I)> | 14.6 | 3.2 |
| Completeness [%] | 91.3 | 72.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 3350, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
