1RX7
STRUCTURE OF DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1995-06 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.990, 45.308, 101.138 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.181 * |
Rwork | 0.181 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ra2 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.980 |
Data reduction software | UCSD |
Data scaling software | UCSD |
Phasing software | MERLOT |
Refinement software | TNT (5D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.480 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.175 * | |
Total number of observations | 13988 * | |
Number of reflections | 6569 | |
<I/σ(I)> | 8.3 | 2.1 |
Completeness [%] | 87.0 | 81 |
Redundancy | 2.1 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | macro seeding * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | cacodylate | 20 (mM) | |
2 | 1 | reservoir | PEG6000 | 20 (%(w/v)) | |
3 | 1 | reservoir | 300 (mM) | ||
4 | 1 | reservoir | cacodylate | 20 (mM) |