1RWE
Enhancing the activity of insulin at receptor edge: crystal structure and photo-cross-linking of A8 analogues
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-08-19 |
Detector | ADSC |
Spacegroup name | H 3 |
Unit cell lengths | 79.341, 79.341, 34.708 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.180 - 1.800 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1TRZ AND 1MPJ |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.180 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.224 |
Number of reflections | 7426 | |
<I/σ(I)> | 23.52 | 9.2 |
Completeness [%] | 98.4 | 95.4 |
Redundancy | 4.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 298 | Tris, sodium citrate, acetone, phenol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.80 |