1RV8

Class II fructose-1,6-bisphosphate aldolase from Thermus aquaticus in complex with cobalt

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Wavelength(s)	0.9791, 0.9783, 0.93, 1.105
Spacegroup name	P 1 2 1
Unit cell lengths	99.354, 57.629, 138.577
Unit cell angles	90.00, 90.23, 90.00

Refinement procedure

Resolution	40.400 * - 2.300
R-factor	0.211
Rwork	0.211
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	22.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.075 *	0.195 *
Total number of observations	989324 *
Number of reflections	68760
Completeness [%]	97.7 *	93.8 *
Redundancy	14.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	295 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.75 (mg/ml)
2	1	drop	sucrose monolaurate	2 (mM)
3	1	reservoir	ammonium sulfate	1.7 (M)
4	1	reservoir	Tris-HCl	0.1 (M)	pH7.5
5	1	reservoir		10 (mM)