1RV6
Crystal Structure of PlGF in Complex with Domain 2 of VEGFR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.281, 71.979, 115.346 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 |
| R-factor | 0.19843 |
| Rwork | 0.194 |
| R-free | 0.26000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.07) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.046 * | 0.161 * |
| Total number of observations | 72785 * | |
| Number of reflections | 15482 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 3 | 1 | reservoir | 0.02 (%) | ||
| 4 | 1 | reservoir | BTP | 0.1 (M) | pH6.5 |
| 5 | 1 | drop | protein | 6.5 (mg/ml) |
