1RTU
USTILAGO SPHAEROGENA RIBONUCLEASE U2
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY |
| Synchrotron site | Photon Factory |
| Temperature [K] | 284 |
| Detector technology | IMAGE PLATE |
| Collection date | 1990-05-22 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.320, 61.270, 34.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-free | 0.17800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RNASE T1 (PDB ENTRY 1RNT) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.028 |
| Data reduction software | TOMOKO |
| Data scaling software | A (PROGRAM BY TOMOKO) |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 * | 0.077 |
| Number of reflections | 9903 | |
| Completeness [%] | 96.1 | 86.4 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.5 | 20 * | CRYSTALS WERE PREPARED BY HANGING-DROP VAPOUR-DIFFUSION METHOD AT 293 KELVIN FROM A 20 MG/ML PROTEIN SOLUTION CONTAINING 15 MG/ML 2'-DEOXY 2'-FLUORO ADENYLYL-3',5'-CYTIDINE, 0.4M AMMONIUM SULFATE, EQUILIBRATED AGAINST A RESERVOIR OF 0.9M AMMONIUM SULFATE CONTAINING 0.1M ACETATE BUFFER (PH 4.5)., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 2'-deoxy-2'-fluro ApC | 1.5 (mg/ml) | |
| 3 | 1 | drop | ammonium sulfate | 0.9 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.9 (M) | |
| 5 | 1 | reservoir | acetate | 0.1 (M) |
