1RTJ

MECHANISM OF INHIBITION OF HIV-1 REVERSE TRANSCRIPTASE BY NON-NUCLEOSIDE INHIBITORS

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1994-11-11
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	137.500, 109.400, 72.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.350
R-factor	0.219
Rwork	0.219
RMSD bond length	0.016
RMSD bond angle	25.000 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.350
Rmerge	0.089
Total number of observations	369013 *
Number of reflections	41307
Completeness [%]	89.5
Redundancy	8.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3400	6 (%)
2	1	reservoir	citrate/phosphate