1RTH
HIGH RESOLUTION STRUCTURES OF HIV-1 RT FROM FOUR RT-INHIBITOR COMPLEXES
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-03-03 |
| Detector | FUJI |
| Wavelength(s) | 0.970 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 141.100, 110.900, 73.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 25.300 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.144 |
| Total number of observations | 455375 * |
| Number of reflections | 48144 |
| Completeness [%] | 81.4 |
| Redundancy | 9.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3400 | 6 (%) | |
| 2 | 1 | reservoir | citrate/phosphate |
