1RT6

HIV-1 REVERSE TRANSCRIPTASE COMPLEXED WITH UC38

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	137.000, 109.300, 71.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.234 *
Rwork	0.236
R-free	0.33500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RT-MKC-442 COMPLEX (PDB ENTRY 1RT1)
RMSD bond length	0.008
RMSD bond angle	24.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.069	0.357
Total number of observations	84517 *
Number of reflections	26073	2446 *
<I/σ(I)>	13.9	1.91
Completeness [%]	95.7	91.3
Redundancy	3.24	2.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5	4 *	pH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	citric acid	24.25 (mM)
2	1	1	sodium phosphate	51.5 (mM)
3	1	1	PEG3400	6-10 (%(w/v))