1RRG
NON-MYRISTOYLATED RAT ADP-RIBOSYLATION FACTOR-1 COMPLEXED WITH GDP, DIMERIC CRYSTAL FORM
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1994-08-30 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.360, 45.010, 91.960 |
Unit cell angles | 90.00, 133.62, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.31500 |
RMSD bond length | 0.011 |
RMSD bond angle | 2.010 |
Data reduction software | MOSFLM |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.081 |
Total number of observations | 40351 * |
Number of reflections | 13030 |
Completeness [%] | 90.0 |
Redundancy | 3.01 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | protein solution is mixed in a 1:1 ratio with well solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 22 (%) | |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | MES | 0.1 (M) |