1RRA

RIBONUCLEASE A FROM RATTUS NORVEGICUS (COMMON RAT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1993-07
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	69.130, 69.130, 60.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.186 *
Rwork	0.186
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rsa
RMSD bond length	0.012
RMSD bond angle	26.180 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.106 *	0.156 *
Number of reflections	4534
Completeness [%]	77.0	60 *
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		35% PEG-8K IN 10MM ACETATE BUFFER PH 5.5 CONTAINING 1MM DISODIUM HYDROGEN PHOSPHATE AND 10 MG/ML PROTEIN

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	acetate	10 (mM)
3	1	drop	disodium hydrogen phosphate	1 (mM)
4	1	reservoir	PEG8000	35 (%)