1RPJ
CRYSTAL STRUCTURE OF D-ALLOSE BINDING PROTEIN FROM ESCHERICHIA COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.380, 67.820, 53.590 |
Unit cell angles | 90.00, 96.71, 90.00 |
Refinement procedure
Resolution | 29.000 - 1.800 |
R-factor | 0.194 * |
Rwork | 0.194 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dri |
RMSD bond length | 0.010 |
RMSD bond angle | 0.026 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.787 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.045 | 0.150 |
Number of reflections | 21163 | |
<I/σ(I)> | 10.4 | 5 |
Completeness [%] | 96.5 | 93.6 |
Redundancy | 2.8 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 550 | ||
2 | 1 | 1 | ZnSO4 | ||
3 | 1 | 1 | MES BUFFER |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | reservoir | mPEG550 | 40-60 (%(v/v)) | |
4 | 1 | reservoir | zinc sulfate | 0.01 (M) | |
5 | 1 | reservoir | MES | 0.1 (M) |