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1ROZ

Deoxyhypusine synthase holoenzyme in its low ionic strength, high pH crystal form

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9B
Synchrotron siteNSLS
BeamlineX9B
Temperature [K]95
Detector technologyCCD
Collection date1999-06-17
DetectorADSC QUANTUM 4
Wavelength(s)0.98
Spacegroup nameP 32 2 1
Unit cell lengths104.780, 104.780, 159.670
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution35.000 - 2.210
Rwork0.178
R-free0.19990
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1dhs
RMSD bond length0.010
RMSD bond angle1.400
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]35.0002.290
High resolution limit [Å]2.2102.210
Number of reflections50963
<I/σ(I)>9.7
Completeness [%]99.999.3
Redundancy3.853.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8298Tris-HCl, KCl, NAD, 2-methyl-2,4-pentanediol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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