1ROZ
Deoxyhypusine synthase holoenzyme in its low ionic strength, high pH crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 1999-06-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 104.780, 104.780, 159.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 - 2.210 |
Rwork | 0.178 |
R-free | 0.19990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dhs |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.290 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 50963 | |
<I/σ(I)> | 9.7 | |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 3.85 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Tris-HCl, KCl, NAD, 2-methyl-2,4-pentanediol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |