1RNN

RIBONUCLEASE A COMPLEX WITH CYTIDYLIC ACID (5'CMP) CRYSTALLIZED FROM 8M SODIUM FORMATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1993
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 32 2 1
Unit cell lengths	65.114, 65.114, 65.272
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.17
Rwork	0.170
RMSD bond length	0.011
RMSD bond angle	2.580
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.041	0.261
Number of reflections	13300
<I/σ(I)>		3.93
Completeness [%]	87.3	54.7
Redundancy	2.52	1.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		pH 5.5