1RNH
STRUCTURE OF RIBONUCLEASE H PHASED AT 2 ANGSTROMS RESOLUTION BY MAD ANALYSIS OF THE SELENOMETHIONYL PROTEIN
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.790, 86.340, 36.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.198 |
| R-free | 0.19800 * |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.5 (%sat) | |
| 3 | 1 | drop | HEPES | 10 (mM) | r |
| 4 | 1 | reservoir | PEG3350 | 20-25 (%(w/w)) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.5 (%sat) | |
| 6 | 1 | reservoir | EDTA | 1 (mM) | |
| 7 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 8 | 1 | reservoir | Tris-HCl | 10 (mM) |
