1RNH
STRUCTURE OF RIBONUCLEASE H PHASED AT 2 ANGSTROMS RESOLUTION BY MAD ANALYSIS OF THE SELENOMETHIONYL PROTEIN
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.790, 86.340, 36.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.198 |
R-free | 0.19800 * |
RMSD bond length | 0.015 |
RMSD bond angle | 0.034 |
Refinement software | PROLSQ |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 1.5 (%sat) | |
3 | 1 | drop | HEPES | 10 (mM) | r |
4 | 1 | reservoir | PEG3350 | 20-25 (%(w/w)) | |
5 | 1 | reservoir | ammonium sulfate | 1.5 (%sat) | |
6 | 1 | reservoir | EDTA | 1 (mM) | |
7 | 1 | reservoir | dithiothreitol | 1 (mM) | |
8 | 1 | reservoir | Tris-HCl | 10 (mM) |