1RNE

THE CRYSTAL STRUCTURE OF RECOMBINANT GLYCOSYLATED HUMAN RENIN ALONE AND IN COMPLEX WITH A TRANSITION STATE ANALOG INHIBITOR

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	90.900, 90.900, 109.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.400
R-factor	0.176
RMSD bond length	0.019 *
RMSD bond angle	3.000 *
Phasing software	X-PLOR
Refinement software	CORELS

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.078 *
Total number of observations	57856 *
Number of reflections	18418 *
Completeness [%]	99.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	3 *		drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	renin	6.3 (mg/ml)
2	1	drop	inhibitor	3 (mg/ml)
3	1	reservoir	ammonium sulfate	1.1-1.3 (M)
4	1	reservoir	sodium citrate	100 (mM)