1RM8
Crystal structure of the catalytic domain of MMP-16/MT3-MMP: Characterization of MT-MMP specific features
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-07 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 51.210, 51.210, 149.368 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.600 - 1.800 |
R-factor | 0.20907 |
Rwork | 0.208 |
R-free | 0.23600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Pdb code 1bqq |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.712 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.600 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.083 * | 0.327 |
Total number of observations | 119029 * | |
Number of reflections | 18455 | |
<I/σ(I)> | 5.5 | 2.2 |
Completeness [%] | 96.7 | 91.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Tris-HCl, Sodium/potassium tartrate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Tris-HCl, Sodium/potassium tartrate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 50 (mM) | pH8.5 |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | cdMT3-MMP | 10 (mg/ml) | |
5 | 1 | drop | batimastat | 0.5 (mg/ml) | |
6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.7 |
7 | 1 | reservoir | sodium phosphate-tartrate | 1.1 (M) |