1RKT
Crystal structure of yfiR, a putative transcriptional regulator from Bacillus subtilis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2003-08-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.983, 48.765, 57.233 |
| Unit cell angles | 71.96, 70.65, 80.20 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.21047 |
| Rwork | 0.208 |
| R-free | 0.25417 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.947 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.045 | 0.279 |
| Number of reflections | 33929 | |
| <I/σ(I)> | 16.318 | 2.6 |
| Completeness [%] | 88.4 | |
| Redundancy | 4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 10mM CaCl2, 0.1M MES, 30% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.8 |
