1RKE

Human vinculin head (1-258) in complex with human vinculin tail (879-1066)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Detector technology	CCD
Wavelength(s)	0.9797
Spacegroup name	P 21 21 2
Unit cell lengths	86.603, 161.648, 36.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.350
Rwork	0.206
R-free	0.27800
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	0.979
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SHARP
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	99.800
High resolution limit [Å]	2.350
Number of reflections	21798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	293	15% PEG 3350; 50 mM NaCl; 100 mM Tris (pH 8); 10 mM DTT, pH 8.0, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	15 (%)
2	1	reservoir		50 (mM)
3	1	reservoir	Tris	100 (mM)	pH8
4	1	reservoir	dithiothreitol	10 (mM)