1RK4
Crystal Structure of a Soluble Dimeric Form of Oxidised CLIC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-01 |
Detector | MAC Science DIP-2030 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.861, 69.189, 107.506 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.740 - 1.792 |
R-factor | 0.17765 |
Rwork | 0.176 |
R-free | 0.22800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k0m |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.440 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.740 | |
High resolution limit [Å] | 1.800 * | 1.800 * |
Rmerge | 0.058 | 0.260 * |
Total number of observations | 163232 * | |
Number of reflections | 40808 * | |
<I/σ(I)> | 18.53 | |
Completeness [%] | 95.3 * | 85.1 * |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 4 * | PEG MME 5000, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.6 (mg/ml) | |
2 | 1 | reservoir | PEG5000 MME | 14 (%(w/v)) | |
3 | 1 | reservoir | sodium acetate | 0.1 (%) | pH4.5 |