1RJX
Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 158.396, 158.396, 158.396 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.725 - 2.300 |
| Rwork | 0.194 |
| R-free | 0.24600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ddj |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.730 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.380 |
| High resolution limit [Å] | 2.320 * | 2.320 * |
| Rmerge | 0.129 | 0.860 |
| Number of reflections | 15398 | 1507 * |
| <I/σ(I)> | 22.4 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | Li2SO4, Na citrate, pH 5.6, hanging drop, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | urea | 0.4 (M) | |
| 4 | 1 | reservoir | 1.0 (M) | ||
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
