1RJX

Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-02-15
Detector	MARRESEARCH
Spacegroup name	I 4 3 2
Unit cell lengths	158.396, 158.396, 158.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.725 - 2.300
Rwork	0.194
R-free	0.24600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ddj
RMSD bond length	0.011 *
RMSD bond angle	1.730 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.380
High resolution limit [Å]	2.320 *	2.320 *
Rmerge	0.129	0.860
Number of reflections	15398	1507 *
<I/σ(I)>	22.4	3
Completeness [%]	100.0	100
Redundancy	14	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	20 *	Li2SO4, Na citrate, pH 5.6, hanging drop, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH8.0
3	1	drop	urea	0.4 (M)
4	1	reservoir		1.0 (M)
5	1	reservoir	sodium citrate	0.1 (M)	pH5.6