1RJB

Crystal Structure of FLT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2003-05-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	80.651, 80.651, 150.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.260 - 2.100
R-factor	0.22
Rwork	0.210
R-free	0.24600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	1.590 *
Data reduction software	HKL-2000
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	AMoRE
Refinement software	CNX (2000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.056	0.265 *
Number of reflections	29690 *
<I/σ(I)>	18.6
Completeness [%]	99.3 *	94.1
Redundancy	6.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	sodium phosphate, potassium phosphate, CAPS, lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium phosphate	1.2 (M)
2	1	reservoir	potassium phosphate	1.2 (M)
3	1	reservoir	CAPS	1.2 (M)
4	1	reservoir	lithium sulfate	0.2 (M)
5	1	drop	protein	10 (mg/ml)
6	1	drop	AMP-PNP	2 (mM)
7	1	drop		4 (mM)