1RJ9

Structure of the heterodimer of the conserved GTPase domains of the Signal Recognition Particle (Ffh) and Its Receptor (FtsY)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	75.040, 83.680, 94.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.239
Rwork	0.206
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	the structures of isolated FtsY and Ffh NG domain
RMSD bond length	0.005
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.077	0.767 *
Total number of observations	305159 *
Number of reflections	47344 *
<I/σ(I)>	16.9	3.1
Completeness [%]	99.3 *	97.9 *
Redundancy	6.3	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	4 *	PEG 8000, Hepes, NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%)
2	1	reservoir	HEPES	100 (mM)	pH7.5